PLP 6241C/4242C.   Bacterial Plant Pathogens

Spring Semester, 2004

 

Instructor:  J. B. Jones (jbjones@ufl.edu)

 

Laboratory Supervisors:  Ellen Dickstein (erd@mail.ifas.ufl.edu)

Jerry Minsavage (gvm@mail.ifas.ufl.edu)

 

Teaching Assistants:        Jason Hong (jchong@ufl.edu)

Aaron Hert (ahert@mail.ifas.ufl.edu)

 

 

Syllabus

 

Lecture Schedule

 

Laboratory Schedule

 

 

Lecture Schedule

            Date

 

Topic

January

   9

Introduction

 

11

Bacterial Structure, Growth

 

18

History, species concept

 

23

Nomenclature

 

25

Taxonomy and Characteristics – Gram – strict aerobes AgrobacteriumI

 

30

Gram negative strict aerobes (continued) - Pseudomonas, Xanthomonas

February

1

Test1 Test2

 

6

Gram negative strict aerobes (continued) - Xylella and  Xylophilus, Rhizomonas

 

8

Gram negative facultative anaerobes – Erwinia, Pantoea, Brenneria, Pectobacterium

 

13

Wall less prokaryotes - Phytoplasma

 

15

Gram + pathogens

 

20

Test1  Test2

 

22

Survival

 

27

Dissemination and Ingress

March

1

Population dynamics

 

6

Hypersensitive reaction

 

8

Susceptible reaction

 

13

Spring Break

 

15

Spring Break

 

20

Test1 Test2

 

22

Extracellular polysaccharides and toxins

 

27

Cell wall degrading enzymes, Growth regulators

 

29

Crown gall

April

  3

Bacterial genetics Cloning made simple

 

  5

Test1, Test2

 

10

hrp genes

 

12

Avirulence genes

 

17

Gene regulation

 

19

Chemical control

 

24

Biological control

 

 

Laboratory Schedule

            Date

Topic

January           

 

11

Isolation of bacteria from plants

Purification and Storage

 

 

18

Physiological tests

 

 

25

Fatty acid profiles, BIOLOG, 16S rRNA sequencing, RiboPrinter

 

February

 

1

DNA extraction, detection by PCR, pulsed-gel electrophoresis

 

 

 8       

Bacteriophages + bacteriocins

 

 

15

 

Standardization of inoculum + Pathogenicity tests

 

 

22

 

Dilution plating, electrolyte leakage, serology

 

March

 1

 

Conjugation + Calculation of mutation rate, Plasmid profiles

 

 

 8

 

Unknowns - Lab instructions

 

 

15       

Spring Break

 

 

22

 

Unknowns

 

 

 

 

29

 

Unknowns

April

  5

 

Unknowns

 

12

Unknowns

 

 

19

Presentations on unknowns

 

 

 26

Presentations on unknowns