PLP 6241C/4242C. Bacterial Plant Pathogens
Spring Semester, 2004
Instructor: J. B. Jones (jbjones@ufl.edu)
Laboratory Supervisors: Ellen Dickstein (erd@mail.ifas.ufl.edu)
Jerry Minsavage (gvm@mail.ifas.ufl.edu)
Teaching Assistants: Jason Hong
(jchong@ufl.edu)
Aaron Hert (ahert@mail.ifas.ufl.edu)
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Date |
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Topic |
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January |
9 |
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11 |
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18 |
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23 |
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25 |
Taxonomy and Characteristics – Gram – strict
aerobes AgrobacteriumI |
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30 |
Gram negative
strict aerobes (continued) - Pseudomonas,
Xanthomonas
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February |
1 |
Test1 Test2 |
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6 |
Gram negative strict aerobes (continued) - Xylella and Xylophilus,
Rhizomonas |
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8 |
Gram negative
facultative anaerobes – Erwinia, Pantoea, Brenneria, Pectobacterium |
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13 |
Wall less prokaryotes - Phytoplasma |
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15 |
Gram + pathogens |
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20 |
Test1 Test2 |
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22 |
Survival |
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27 |
Dissemination and Ingress |
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March |
1 |
Population dynamics |
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6 |
Hypersensitive reaction |
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8 |
Susceptible reaction |
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13 |
Spring Break |
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15 |
Spring Break |
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20 |
Test1 Test2 |
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22 |
Extracellular polysaccharides and toxins |
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27 |
Cell wall degrading enzymes, Growth regulators |
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29 |
Crown gall |
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April |
3 |
Bacterial genetics Cloning made simple |
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5 |
Test1, Test2 |
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10 |
hrp genes |
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12 |
Avirulence genes |
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17 |
Gene regulation |
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19 |
Chemical control |
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24 |
Biological control |
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Date |
Topic |
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January
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11 |
Isolation of bacteria from plants Purification and Storage
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18 |
Physiological tests
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25 |
Fatty acid profiles, BIOLOG, 16S rRNA sequencing, RiboPrinter
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February
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1 |
DNA extraction, detection by PCR, pulsed-gel electrophoresis
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8 |
Bacteriophages + bacteriocins |
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15
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Standardization of inoculum + Pathogenicity tests
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22
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Dilution plating, electrolyte leakage, serology
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March |
1
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Conjugation + Calculation of mutation rate, Plasmid profiles
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8
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Unknowns - Lab instructions |
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15 |
Spring Break |
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22 |
Unknowns |
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29
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Unknowns |
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April |
5
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Unknowns |
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12 |
Unknowns
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19 |
Presentations on unknowns
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26 |
Presentations on unknowns |
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